S4 class that holds parameters for the Lun2 simulation.
The Lun2 simulation uses the following parameters:
nGenes
The number of genes to simulate.
nCells
The number of cells to simulate.
[seed]
Seed to use for generating random numbers.
gene.params
A data.frame
containing gene
parameters with two columns: Mean
(mean expression for
each gene) and Disp
(dispersion for each gene).
zi.params
A data.frame
containing
zero-inflated gene parameters with three columns: Mean
(mean expression for each gene), Disp
(dispersion for
each, gene), and Prop
(zero proportion for each gene).
[nPlates]
The number of plates to simulate.
plate.ingroup
Character vector giving the plates considered to be part of the "ingroup".
plate.mod
Plate effect modifier factor. The plate effect variance is divided by this value.
plate.var
Plate effect variance.
cell.plates
Factor giving the plate that each cell comes from.
cell.libSizes
Library size for each cell.
cell.libMod
Modifier factor for library sizes. The library sizes are multiplied by this value.
de.nGenes
Number of differentially expressed genes.
de.fc
Fold change for differentially expressed genes.
The parameters not shown in brackets can be estimated from real data using
lun2Estimate
. For details of the Lun2 simulation see
lun2Simulate
.