Generate library sizes for cells in the Kersplat simulation
kersplatSimLibSizes(sim, params, verbose)
| sim | SingleCellExperiment containing simulation. |
|---|---|
| params | KersplatParams object with simulation parameters. |
| verbose | logical. Whether to print progress messages |
SingleCellExperiment with library sizes
Library sizes are simulated in one of two ways depending on the value of the
lib.method parameter.
If lib.method is "fit" (default) then means are sampled from a
log-normal distribution with meanlog equals lib.loc and sdlog equals
lib.scale.
If mean.method is "density" then library sizes are sampled from the
density object in the lib.density parameter using a rejection
sampling method. This approach is more flexible but may violate some
statistical assumptions.
Ambient library sizes are also generated from a log-normal distribution based
on the parameters for the cell library size and adjusted using the
ambient.scale parameter.